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affigel-15 beads  (Bio-Rad)


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    Structured Review

    Bio-Rad affigel-15 beads
    Affigel 15 Beads, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/affigel-15+beads/affigel+10+beads/pm38154460-355-18-20
    Average 90 stars, based on 1 article reviews
    affigel-15 beads - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: Peroxisome protein import recapitulated in Xenopus egg extracts
    Article Snippet: 4 ml of packed Affigel-15 beads (BioRad) was incubated with 24 mg of purified Pex5 in 30 mM Hepes, pH 7.8, and 100 mM KCl overnight at 4°C.

    Article Title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
    Article Snippet: In the first step, TF was extracted from plasma in 1 vol% Triton by CLB/TF-1 antibody, 92 ª 2005 Blackwell Publishing Ltd, British Journal of Haematology, 131, 91–99 covalently coupled to Affigel-15 beads (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Affinity purification using emerin-conjugated beads and mass spectrometry Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.


    Article Title: Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens.
    Article Snippet: Article Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.

    Article Title: Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis
    Article Snippet: The proteins were coupled to AffiGel-15 beads (Bio-Rad) overnight at 4°C at a concentration of 5 mg/mL of beads.

    Article Title: ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons.
    Article Snippet: Several members of the ADAM (a disintegrin and metalloprotease) family of proteins have been implicated in biological processes ranging from fertilization to myoblast fusion and neural cell fate determination.. These proteins have so far been studied mostly in terms of their protease activity, but a considerable amount of evidence suggests that many ADAMs are also important as receptors for cell-surface integrins.. We have shown that, for one such member of the family, ADAM23, mRNA transcripts are expressed in neuronal cells throughout the rat brain, at all stages of postnatal development, and that particularly high transcript concentrations are found in the hippocampus and cerebellum.

    Affinity Purification:

    Article Title: Peroxisome protein import recapitulated in Xenopus egg extracts
    Article Snippet: 4 ml of packed Affigel-15 beads (BioRad) was incubated with 24 mg of purified Pex5 in 30 mM Hepes, pH 7.8, and 100 mM KCl overnight at 4°C.

    Article Title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
    Article Snippet: In the first step, TF was extracted from plasma in 1 vol% Triton by CLB/TF-1 antibody, 92 ª 2005 Blackwell Publishing Ltd, British Journal of Haematology, 131, 91–99 covalently coupled to Affigel-15 beads (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Affinity purification using emerin-conjugated beads and mass spectrometry Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.


    Article Title: Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens.
    Article Snippet: Article Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.

    Article Title: Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis
    Article Snippet: The proteins were coupled to AffiGel-15 beads (Bio-Rad) overnight at 4°C at a concentration of 5 mg/mL of beads.

    Article Title: ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons.
    Article Snippet: Several members of the ADAM (a disintegrin and metalloprotease) family of proteins have been implicated in biological processes ranging from fertilization to myoblast fusion and neural cell fate determination.. These proteins have so far been studied mostly in terms of their protease activity, but a considerable amount of evidence suggests that many ADAMs are also important as receptors for cell-surface integrins.. We have shown that, for one such member of the family, ADAM23, mRNA transcripts are expressed in neuronal cells throughout the rat brain, at all stages of postnatal development, and that particularly high transcript concentrations are found in the hippocampus and cerebellum.

    Incubation:

    Article Title: Peroxisome protein import recapitulated in Xenopus egg extracts
    Article Snippet: 4 ml of packed Affigel-15 beads (BioRad) was incubated with 24 mg of purified Pex5 in 30 mM Hepes, pH 7.8, and 100 mM KCl overnight at 4°C.

    Article Title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
    Article Snippet: In the first step, TF was extracted from plasma in 1 vol% Triton by CLB/TF-1 antibody, 92 ª 2005 Blackwell Publishing Ltd, British Journal of Haematology, 131, 91–99 covalently coupled to Affigel-15 beads (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Affinity purification using emerin-conjugated beads and mass spectrometry Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.


    Article Title: Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens.
    Article Snippet: Article Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.

    Article Title: Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis
    Article Snippet: The proteins were coupled to AffiGel-15 beads (Bio-Rad) overnight at 4°C at a concentration of 5 mg/mL of beads.

    Article Title: ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons.
    Article Snippet: Several members of the ADAM (a disintegrin and metalloprotease) family of proteins have been implicated in biological processes ranging from fertilization to myoblast fusion and neural cell fate determination.. These proteins have so far been studied mostly in terms of their protease activity, but a considerable amount of evidence suggests that many ADAMs are also important as receptors for cell-surface integrins.. We have shown that, for one such member of the family, ADAM23, mRNA transcripts are expressed in neuronal cells throughout the rat brain, at all stages of postnatal development, and that particularly high transcript concentrations are found in the hippocampus and cerebellum.

    Purification:

    Article Title: Peroxisome protein import recapitulated in Xenopus egg extracts
    Article Snippet: 4 ml of packed Affigel-15 beads (BioRad) was incubated with 24 mg of purified Pex5 in 30 mM Hepes, pH 7.8, and 100 mM KCl overnight at 4°C.

    Article Title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
    Article Snippet: In the first step, TF was extracted from plasma in 1 vol% Triton by CLB/TF-1 antibody, 92 ª 2005 Blackwell Publishing Ltd, British Journal of Haematology, 131, 91–99 covalently coupled to Affigel-15 beads (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Affinity purification using emerin-conjugated beads and mass spectrometry Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.


    Article Title: Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens.
    Article Snippet: Article Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.

    Article Title: Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis
    Article Snippet: The proteins were coupled to AffiGel-15 beads (Bio-Rad) overnight at 4°C at a concentration of 5 mg/mL of beads.

    Article Title: ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons.
    Article Snippet: Several members of the ADAM (a disintegrin and metalloprotease) family of proteins have been implicated in biological processes ranging from fertilization to myoblast fusion and neural cell fate determination.. These proteins have so far been studied mostly in terms of their protease activity, but a considerable amount of evidence suggests that many ADAMs are also important as receptors for cell-surface integrins.. We have shown that, for one such member of the family, ADAM23, mRNA transcripts are expressed in neuronal cells throughout the rat brain, at all stages of postnatal development, and that particularly high transcript concentrations are found in the hippocampus and cerebellum.

    Mass Spectrometry:

    Article Title: Peroxisome protein import recapitulated in Xenopus egg extracts
    Article Snippet: 4 ml of packed Affigel-15 beads (BioRad) was incubated with 24 mg of purified Pex5 in 30 mM Hepes, pH 7.8, and 100 mM KCl overnight at 4°C.

    Article Title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
    Article Snippet: In the first step, TF was extracted from plasma in 1 vol% Triton by CLB/TF-1 antibody, 92 ª 2005 Blackwell Publishing Ltd, British Journal of Haematology, 131, 91–99 covalently coupled to Affigel-15 beads (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Affinity purification using emerin-conjugated beads and mass spectrometry Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.


    Article Title: Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens.
    Article Snippet: Article Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.

    Article Title: Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis
    Article Snippet: The proteins were coupled to AffiGel-15 beads (Bio-Rad) overnight at 4°C at a concentration of 5 mg/mL of beads.

    Article Title: ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons.
    Article Snippet: Several members of the ADAM (a disintegrin and metalloprotease) family of proteins have been implicated in biological processes ranging from fertilization to myoblast fusion and neural cell fate determination.. These proteins have so far been studied mostly in terms of their protease activity, but a considerable amount of evidence suggests that many ADAMs are also important as receptors for cell-surface integrins.. We have shown that, for one such member of the family, ADAM23, mRNA transcripts are expressed in neuronal cells throughout the rat brain, at all stages of postnatal development, and that particularly high transcript concentrations are found in the hippocampus and cerebellum.

    Negative Control:

    Article Title: Peroxisome protein import recapitulated in Xenopus egg extracts
    Article Snippet: 4 ml of packed Affigel-15 beads (BioRad) was incubated with 24 mg of purified Pex5 in 30 mM Hepes, pH 7.8, and 100 mM KCl overnight at 4°C.

    Article Title: State and diagnostic value of plasma tissue factor in early-hospitalised patients with chest pain.
    Article Snippet: In the first step, TF was extracted from plasma in 1 vol% Triton by CLB/TF-1 antibody, 92 ª 2005 Blackwell Publishing Ltd, British Journal of Haematology, 131, 91–99 covalently coupled to Affigel-15 beads (Bio-Rad Laboratories, Hercules, CA, USA).

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Affinity purification using emerin-conjugated beads and mass spectrometry Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.


    Article Title: Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens.
    Article Snippet: Article Extracellular carriers control lipid-dependent secretion, delivery, and activity of WNT morphogens

    Article Title: An emerin 'proteome': purification of distinct emerin-containing complexes from HeLa cells suggests molecular basis for diverse roles including gene regulation, mRNA splicing, signaling, mechano-sensing and nuclear architecture
    Article Snippet: Wildtype emerin residues 1-222 or BSA (negative control) were coupled to Affigel-15 beads (Bio-Rad Laboratories) per manufacturer instructions.

    Article Title: Advanced Proteomic Analyses Yield a Deep Catalog of Ubiquitylation Targets in Arabidopsis
    Article Snippet: The proteins were coupled to AffiGel-15 beads (Bio-Rad) overnight at 4°C at a concentration of 5 mg/mL of beads.

    Article Title: ADAM23 is a cell-surface glycoprotein expressed by central nervous system neurons.
    Article Snippet: Several members of the ADAM (a disintegrin and metalloprotease) family of proteins have been implicated in biological processes ranging from fertilization to myoblast fusion and neural cell fate determination.. These proteins have so far been studied mostly in terms of their protease activity, but a considerable amount of evidence suggests that many ADAMs are also important as receptors for cell-surface integrins.. We have shown that, for one such member of the family, ADAM23, mRNA transcripts are expressed in neuronal cells throughout the rat brain, at all stages of postnatal development, and that particularly high transcript concentrations are found in the hippocampus and cerebellum.



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